Specificity: Rabbit polyclonal
antibody against Human SUMO I (Ubiquitin-like protein
Antigen used for
immunizations: Prokaryotic recombinant
protein corresponding to the full expressed region
of the human Ubiquitin-like
protein SMT3C precursor [access no: Q93068, also called
Ubiquitin-homology domain protein PIC1,
Ubiquitin-like protein UBL1, Ubiquitin-related
protein SUMO-1, GAP modifying protein 1, GMP1, and
was stored in lyophilized
Before it is used, it should be reconstitited with
100 ul of H2O. Working dilution on
Western blotting is
Recommendations on use:
Quality of anti-SUMO I antibody
The quality of anti-SUMO I antibody at 1,000-fold dilution is good to detect SUMO I at the concentration
as low as 5 ng of SUMO I. SUMO I was run on a 15% SDS-PAGE, followed by transferring to PVDF membrane and
immunoblotting with 1,000-fold diluted.
Lane 1, protein marker
Lane 2, 5 ng; Lane 3, 10 ng; Lane 4, 25 ng; Lane 5, 50 ng;
Lane 6, 100 ng; Lane 7, 200 ng of SUMO I.
Western blots (12.5% SDS-PAGE) of sumoylated 50Kd-Topo
I substrate with anti-topo I antibody (AP006) and anti-SUMO I antibody (AP018)
Lane 1 & 2 are blotted with 1,000-fold diluted anti-topo I antibody (AP006).
Lane 3 & 4 are blotted with 1,000-fold diluted anti-SUMO I antibody (AP018).
Lane 1 and Lane 3 are 50Kd-Topo I substrate (B007).
Lane 2 and Lane 4 are sumoylated 50Kd-Topo I substrate which are obtained by incubating 2
ml of 50Kd-Topo I substrate with 2
ml E1 (P001), 2
ml of E2 (P002), 2
ml of SUMO I (P003), 2
ml of 10X sumoylation buffer (B006), 2
ml of 20 mM ATP and 8
ml of H2O at 37oC for 45 min.
Storage and stability:
Store unopened antibody at
4oC or lower. Under these conditions, there is no
significant loss in product performance
to one year from the purchase date. The reconstituted
antibody is stable for at least two months when
stored at 4oC. For long term storage, it is
recommended that aliquots of the antibody are
frozen at -20oC (frost-free freezers are not
recommended). Repeated freezing and thawing must
be avoided. Prepare working dilutions on the day
consideration: FOR RESEARCH USE