(Catalog # P003;
The active human SUMO I (the 1-97 animo acid region of the
Ubiquitin-like protein SMT3C precursor
[access no: Q93068,
also called Ubiquitin-homology domain protein PIC1, Ubiquitin-like
protein UBL1, Ubiquitin-related protein SUMO-1, GAP modifying protein 1, GMP1,
is produced from
a E. coli expression system, prepared from freshly extracted cell lysates, and
purified to homogeneity using methods developed by LAE Biotechnology Co., LTD.
The final fraction of enzyme contains a single polypeptide band of 11 kDa.
LAE-SUMO I is supplied at the concentration of 0.5ug/ul
(Cat # P003:
Cat # P012: 50ug/tube, 100 ul/tube).
The recommended working concentration is 50ug/ml.
final fraction of LAE-SUMO I is an affinity column pool and is in the following
buffer: 10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM
DTT, and 10 % glycerol. The enzyme is stable in this buffer at -20~70 oC. In
general, the enzyme is less stable when diluted. We doníŽt recommend any dilution
of the enzyme.
The enzyme is shipped on
ice and must be stored at -20oC or lower.
consideration: FOR RESEARCH
Figure: TOPO I sumoylation with
sumo-1 and sumo-3
Right Lane: Topo I protein (TopoI:
Middle Lane: Sumo-I sumoylated
Topo I (Sumo-I: Cat# P003; TopoI: Cat# B007; EI: Cat# P001; EII: Cat# P002;
Buffer: Cat# B006)
Left Lane: Sumo-3 sumoylated Topo
I (Sumo-3: Cat# P004; TopoI: Cat# B007; EI: Cat# P001; EII: Cat# P002; Buffer:
Note: Topo I is S35-Met