SUMO III, active
(Catalog # P004;
The active human SUMO III (Ubiquitin-like protein SMT3B
[access no: P55855]) is produced from a E. coli expression system, prepared from freshly extracted cell
lysates, and purified to homogeneity using methods developed by LAE
Biotechnology Co., LTD. The final fraction of enzyme contains a single
polypeptide band of 11 kDa.
LAE-SUMO III is supplied at the concentration of 0.5ug/ul
(Cat # P004: 100 ug/tube, 200 ul/tube; Cat # P013: 50 ug/tube, 100 ul/tube).
The recommended working condition is 1 ug of SUMO III in 20 ul of reaction mixtures.
The final fraction of LAE-SUMO III is an affinity column pool and is in the following buffer:
10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1
mM DTT, and 10 % glycerol. The enzyme is stable in this buffer at -20 oC
to -70 oC. In general, the enzyme is less stable when diluted. We do not recommend
any dilution of the enzyme.
The enzyme is shipped on ice and should be stored at -20oC or lower.
consideration: FOR RESEARCH
Figure: TOPO I sumoylation with
sumo-1 and sumo-3
Right Lane: Topo I protein (TopoI: Cat#
Middle Lane: Sumo-I sumoylated Topo I
(Sumo-I: Cat# P003; TopoI: Cat# B007; EI: Cat# P001; EII: Cat# P002; Buffer:
Left Lane: Sumo-3 sumoylated Topo I
(Sumo-3: Cat# P004; TopoI: Cat# B007; EI: Cat# P001; EII: Cat# P002; Buffer:
Note: Topo I is S35-Met