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Human SUMO1(Lys->Arg)

(Catalog # P005; P014)



Description:

Human SUMO1(Lys->Arg) is a mutant of the active human SUMO1 with all the internal lysine residues of SUMO1 replaced with argine residues. Human SUMO1(Lys->Arg) is produced from a E. coli expression system, prepared from freshly extracted cell lysates, and purified to homogeneity using methods developed by LAE Biotechnology Co., LTD. The final fraction of enzyme contains a single polypeptide band of 11 kDa.


Concentration:

LAE-human SUMO1(Lys->Arg) is supplied at the concentration of 0.5ug/ul (Cat # P005: 100ug/tube, 200ul/tube; Cat# P014: 50ug/tube, 100ul/tube).

The recommended working concentration is 50ug/ml.


Storage Buffer:

The final fraction of LAE-humanSUMO1(Lys->Arg) is an affinity column pool and is in the following buffer: 10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol. The protein is stable in this buffer at -20~70oC. In general, the protein is less stable when diluted. We doníŽt recommend any dilution of the protein.


Shipping/Storage:

The protein is shipped on ice and must be stored at -20oC or lower.


Legal consideration: FOR RESEARCH USE ONLY.



Figure Legend:

GST-hTOP1110-125 was subjected to in vitro sumoylation in the presence of either wild type SUMO1 or SUMO1(Lys->Arg). The reaction products were analyzed by 12.5 % SDS-polyacrylamide gel, followed by immunoblotting using anti-GST antibodies.