(Catalog # P021)
DARPP-32 stands for dopamine- and cAMP-regulated protein with molecular weight of 32 kDa
(1). DARPP-32 is an important component of dopaminergic function in the brain. It acts as an intracellular third messenger. DARPP-32 can be phosphorylated at Thr34
by cAMP-dependent protein kinase. Phosphorylation of DARPP-32 results in its conversion to a potent inhibitor (IC50 approximately 1 nM) of the catalytic subunit of protein
phosphatase-1 (PP1). Recombinant rat DARPP-32 is produced from E. coli.,
purified from freshly extracted E. coli lysates, and purified to
homogeneity using methods developed by LAE Biotechnology Co., LTD. The final fraction of rat DARPP-32
exhibits a single polypeptide band of 32 kDa.
Quality of Rat DARPP-32:
Recombinant rat DARPP-32 is supplied in lyophilized form at the dose of 100 ug per vail (Catalog # P021)
Lyophilized recombinant rat DARPP-32 should be should be
reconstituted in 100 ul of 50 mM Tris-HCl, pH 7.0 to make a final concentration
of 1 mg/ml.
The recombinant rat DARPP-32 is shipped on ice and must be
stored at -20 C or lower.
Legal consideration: FOR RESEARCH USE ONLY
Recommended long term storage -70 C; avoid repeated freeze/thaw cycles.
Quality Assurance Statement:
Recombinant rat DARPP-32 contains no detectable protease activity. Tests for phosphatase activity showed no
1. Huang, H.-B., Horiuchi, A., Watanabe, T., Shih, S. R., Tsay,
H. J., Li, H. C., Greengard, P., Nairn, A. C. (1999) Characterization of the inhibition of protein phosphastase-1 by
DARPP-32 and inhibitor-2. J. Biol. Chem. 274, 7870-7878.