(Catalog # P022 & P023)
DARPP-32 stands for dopamine- and cAMP-regulated protein with molecular weight of 32 kDa
(1). DARPP-32 is an important component of dopaminergic function in the
brain. It acts as an intracellular third messenger. DARPP-32 can be
phosphorylated at Thr34 by cAMP-dependent protein kinase. Phosphorylation of DARPP-32 results in its
conversion to a potent inhibitor (IC50 approximately 1 nM) of the catalytic
subunit of protein phosphatase-1 (PP1). Recombinant rat DARPP-32 is produced from E. coli., purified from freshly extracted E.
coli lysates, and purified to homogeneity using methods developed by LAE
Biotechnology Co., LTD. The final fraction of rat DARPP-32 exhibits a single polypeptide band of 32
kDa. Phospho-DARPP-32 was prepared by phosphorylation at Thr34 of DARPP-32 by cAMP-dependent
Quality of Rat Phospho-DARPP-32:
Recombinant rat phospho-DARPP-32 is supplied in
lyophilized form at the dose of 100 ug per vail (Catalog # P022) and at
the dose of 50 ug per vail (Catalog # P023)
Lyophilized recombinant rat DARPP-32 (catalog # P022, 100
ug/vail) should be reconstituted in 100 ul of 50 mM Tris-HCl, pH 7.0, while
lyophilized protein phosphatase inhibitor-1 (catalog # P023, 50 ug/vail) should
be reconstituted in 50 ul of 50 mM Tris-HCl, pH 7.0, to make a final
concentration of 1 mg/ml.
The recombinant rat DARPP-32 is shipped on ice and must be
stored at -20 C or lower.
Legal consideration: FOR RESEARCH USE ONLY
Recommended long term storage at -70 C; avoid repeated freeze/thaw cycles.
Quality Assurance Statement:
Recombinant rat phospho-DARPP-32 contains no
detectable protease activity. Tests for phosphatase activity showed no
1. Huang, H.-B., Horiuchi, A., Watanabe, T., Shih, S. R., Tsay, H. J., Li, H. C., Greengard, P., Nairn, A. C.
(1999) Characterization of the inhibition of protein phosphastase-1 by DARPP-32 and inhibitor-2. J. Biol. Chem. 274, 7870-7878.