Protein Phosphatase Inhibitor-1
(Catalog # P024)
Human recombinant protein phosphatase inhibitor-1 is produced from E. coli.,
purified from freshly extracted E. coli lysates, and purified to
homogeneity using methods developed by LAE Biotechnology Co., LTD. The final fraction of protein phosphatase
inhibitor-1 exhibits a single polypeptide band of 27 kDa. Protein phosphatase
inhibitor-1 can be phosphorylated by cAMP-dependent protein kinase. Protein
phosphatase inhibitor-1 after phosorylation on Thr-35 becomes a potent
inhibitor of type 1 protein phosphatase (1, 2). Phospho-inhibitor-1 specifically
inhibits the catalytic subunit of type 1 protein phosphatases (PP1) at
nanomolar concentrations (IC50 ~ 2 nM).
Quality of Protein Phosphatase Inhibitor-1:
1X PP1 Reaction Buffer:
50 mM Tris-HCl, 5 mM dithiothreitol,
0.1 mM EDTA, pH 7.0
1X PP1 Reaction Buffer supplemented with
1 mM MnCl2 and 5 mM Caffeine. Reaction should be performed at 30 C.
Protein phosphatase inhibitor-1 is supplied in
lyophilized form at the dose of 100 ug per vail (Catalog # P024).
Lyophilized protein phosphatase inhibitor-1 should be
reconstituted in 100 ul of 50 mM Tris-HCl, pH 7.0 to make a final concentration
of 1 mg/ml.
The protein phosphatase inhibitor-1 is shipped on ice and must
be stored at -20 C or lower.
Legal consideration:FOR RESEARCH USE ONLY
1. Supplement with 1 mM MnCl2 and 5 mM caffeine
(required only if phosphorylase a is the substrate).
2. Protein phosphatase inhibitor-1 has been purified
to > 95% homogeneity as determined by SDS-PAGE and Coomassie Blue staining.
3. Recommended long term storage -70 C; avoid repeated freeze/thaw cycles.
Quality Assurance Statement:
Protein phosphatase inhibitor-1 contains no detectable protease activity. Tests for phosphatase activity showed no
1. Connor, J. H. (1998) Inhibitor-1 interaction domain that mediates the inhibition of protein phosphatase-1. J. Biol. Chem.
2. Connor, J. H. (1999) Importance of the beta12-beta13 loop in protein phosphatase-1 catalytic subunit for inhibition by
toxins and mammalian protein inhibitors. J. Biol. Chem. 274, 22366.