Epitope design and LAE Immunogen Preparation

4 weeks (USD 1,500)

• Custom linear epitope array synthesis up to 12 amino acids. We can provide assistance on designing peptide with respect to protein homology, immunogenicity, hydrophilicity, and synthetic suitability.
• LAE repeat is then reconstructed to link with GST.
• LAE repeat-GST immunogen is expressed and purified for injection.
• Epitope monomer is also constructed to an unique PE protein from LAE BIO.
• Epitope Monomer-PE is expressed and purified for western blotting analysis and other functional studies.

Immunization and anti-sera production & Test

12 weeks (USD 700)

• LAE repeat-GST immunogen is used to immunize rabbits.
• Pre-immune and three immune bleeds.
• 80-100 ml rabbit antiserum or 10 ml mouse antiserum produced.
• Western blot analysis guarantees to reach target titer.

 Total 16 weeks

(USD 2,200)

• Lyophilized antiserum is supplied.

Peptide design and MAP immunogen synthesis

3 weeks (USD 600)

• Custom MAP peptide synthesis of 15 amino acids. Peptide with more than 15 amino acids will be charged for USD15 per additional residue. We can provide assistance one designing peptide with respect to protein homology, immunogenicity, hydrophilicity, and synthetic suitability.

Conjugation Service

1 week (USD 300)

• Conjugation of peptide (using Cysteine) to BSA/OVA/KLH.

• Using Cysteine residue at the N-terminal of peptide to conjugate for western blotting and other functional studies.

Purification

2 days (USD 400)

• Protein A purification and lyophilization.

Antibody Test

1 week (USD 400)

• Immunohistochemistry (IHC) on tissues or cultured cells.
• Immunoprecipitation (IP) on tissues or cell lysate.