Human GnRH I Antibody

(Catalog # AP009)

Specificity: Rabbit polyclonal antibody against human GnRH I (gonadotropin-releasing hormone I or Luteinizing hormone releasing hormone I).

Antigen used for immunizations: Prokaryotic recombinant protein containing ten copies of the amino acid residues 24 to 33 of the human GnRH I protein in linear array [access no: P01148].

Storage Buffer: The ammonium sulfate-precipitated whole serum is kept in PBS with 1% BSA and then lyophilized.

Recommendations on use:

Immunohistochemistry:

Not recommended.

Western blotting:

        Working dilution on Western blotting 1:1000. 60 minutes primary antibody incubates at 25^oC.

Storage and stability: Store unopened antibody at 4^oC or lower. Under these conditions, there is no significant loss in product performance up to one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4^oC. For long term storage, it is recommended that aliquots of the antibody are frozen at -20^oC (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Legal consideration: FOR RESEARCH USE ONLY.

References:

Seeburg,P.H. and Adelman,J.P. Characterization of cDNA for precursor of human luteinizing hormone releasing hormone. Nature 311 (5987), 666-668 (1984).

Adelman,J.P., Mason,A.J., Hayflick,J.S. and Seeburg,P.H. Isolation of the gene and hypothalamic cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release- inhibiting factor in human and rat. Proc. Natl. Acad. Sci. U.S.A. 83 (1), 179-183 (1986).