Specificity: Rabbit polyclonal
antibody against human ACTH (Adrenocorticotropic hormone).
Antigen used for
immunizations: Prokaryotic recombinant
protein containing eight copies of the amino acid
residues 150 to 161 of the human ACTH protein in
linear array [access no: P01189].
Storage Buffer: The ammonium sulfate-precipitated
is kept in PBS with 1% BSA and then
Recommendations on use:
Working dilution on
blotting 1:1000. 60 minutes primary
antibody incubates at 25oC.
Storage and stability:
Store unopened antibody at
4oC or lower. Under these conditions, there is no
significant loss in product performance
to one year from the purchase date. The reconstituted antibody
is stable for at least two months when stored at
4oC. For long term storage, it is recommended that
aliquots of the antibody are frozen at -20oC
(frost-free freezers are not recommended).
Repeated freezing and thawing must be avoided.
Prepare working dilutions on the day of
consideration: FOR RESEARCH USE
Lee,T.H., Lerner,A.B. and
Buettner-Janusch,V. On the structure of human
corticotropin (adrenocorticotropic hormone). J.
Biol. Chem. 236, 2970-2974
Sieber,P., Rittel,W. and Zuber,H. Revised
amino-acid sequences for porcine and human
adrenocorticotrophic hormone. J. Nature New Biol.
235 (56), 114-115