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Human HER II Receptor Antibody

(Catalog # AP014)

Specificity: Rabbit polyclonal antibody against human HER II receptor (Receptor protein-tyrosine kinase erbB-2 precursor (p185erbB2), Tyrosine kinase-type cell surface receptor HER2).

Antigen used for immunizations: Prokaryotic recombinant protein containing eighteen copies of the amino acid residues 127 to 136 of the human HER II receptor in linear array [access no: P04626].

Storage Buffer: The ammonium sulfate-precipitated whole serum is kept in PBS with 1% BSA and then lyophilized.

Recommendations on use:


Not recommended.

Western blotting:

Working dilution on Western blotting 1:1000. 60 minutes primary antibody incubates at 25oC.

Positive controls: SK-BR3 cells.

Storage and stability: Store unopened antibody at 4oC or lower. Under these conditions, there is no significant loss in product performance up to one year from the purchase date. The reconstituted antibody is stable for at least two months when stored at 4oC. For long term storage, it is recommended that aliquots of the antibody are frozen at -20oC (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

Legal consideration: FOR RESEARCH USE ONLY.


Coussens,L., Yang-Feng,T.L., Liao,Y.C., Chen,E., Gray,A., McGrath,J., Seeburg,P.H., Libermann,T.A., Schlessinger,J., Francke,U., Levinson,A. and Ullrich,A. Tyrosine kinase receptor with extensive homology to

EGF receptor shares chromosomal location with neu oncogene. Science 230 (4730), 1132-1139 (1985).