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Human HER II
Receptor Antibody
(Catalog #
AP014)
Specificity:
Rabbit
polyclonal antibody against human HER II receptor
(Receptor protein-tyrosine kinase erbB-2 precursor
(p185erbB2), Tyrosine kinase-type cell surface
receptor HER2).
Antigen used for
immunizations: Prokaryotic recombinant
protein containing eighteen copies of the amino
acid residues 127 to 136 of the human HER II
receptor in linear array [access no: P04626].
Storage Buffer: The ammonium sulfate-precipitated
whole
serum
is kept in PBS with 1% BSA and then
lyophilized.
Recommendations on use:
Immunohistochemistry:
Not
recommended.
Western blotting:
Working dilution on
Western
blotting 1:1000. 60 minutes primary
antibody incubates at
25oC.
Positive controls:
SK-BR3
cells.
Storage and stability:
Store unopened antibody at
4oC or lower. Under these conditions, there is no
significant loss in product performance
up
to one year from the purchase date. The reconstituted antibody
is stable for at least two months when stored at
4oC. For long term storage, it is recommended that
aliquots of the antibody are frozen at -20oC
(frost-free freezers are not recommended).
Repeated freezing and thawing must be avoided.
Prepare working dilutions on the day of
use.
Legal
consideration: FOR RESEARCH USE
ONLY.
References:
Coussens,L.,
Yang-Feng,T.L., Liao,Y.C., Chen,E., Gray,A.,
McGrath,J., Seeburg,P.H., Libermann,T.A.,
Schlessinger,J., Francke,U., Levinson,A. and
Ullrich,A. Tyrosine kinase receptor with extensive
homology to
EGF receptor
shares chromosomal location with neu oncogene.
Science 230 (4730), 1132-1139 (1985). | 
