Human SUMO1(Lys->Arg)

(Catalog # P005; P014)

Description:

Human SUMO1(Lys->Arg) is a mutant of the active human SUMO1 with all the internal lysine residues of SUMO1 replaced with argine residues. Human SUMO1(Lys->Arg) is produced from a E. coli expression system, prepared from freshly extracted cell lysates, and purified to homogeneity using methods developed by LAE Biotechnology Co., LTD. The final fraction of enzyme contains a single polypeptide band of 11 kDa.

Concentration:

LAE-human SUMO1(Lys->Arg) is supplied at the concentration of 0.5ug/ul (Cat # P005: 100ug/tube, 200ul/tube; Cat# P014: 50ug/tube, 100ul/tube).

The recommended working concentration is 50ug/ml.

Storage Buffer:

The final fraction of LAE-humanSUMO1(Lys->Arg) is an affinity column pool and is in the following buffer: 10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol. The protein is stable in this buffer at -20~70^oC. In general, the protein is less stable when diluted. We don’t recommend any dilution of the protein.

Shipping/Storage:

The protein is shipped on ice and must be stored at -20^oC or lower.

Legal consideration: FOR RESEARCH USE ONLY.

Figure Legend:

GST-hTOP1^110-125 was subjected to in vitro sumoylation in the presence of either wild type SUMO1 or SUMO1(Lys->Arg). The reaction products were analyzed by 12.5 % SDS-polyacrylamide gel, followed by immunoblotting using anti-GST antibodies.