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Human UBC9[1]
(Catalog # P002; P011)

 

Description:
Human UBC9 (Ubiquitin-like protein SUMO-1 conjugating enzyme [access no: P50550])is produced from a E. coli expression system, prepared from freshly extracted cell lysates, and purified to homogeneity using methods developed by LAE Biotechnology Co., LTD. The final fraction of enzyme contains a single polypeptide band of 18 kDa.

Storage Buffer:
The final fraction of the LAE-E II-SUMO is an affinity column pool and is in the following buffer: 10 mM Tris-Cl, pH 7.5, 100 mM NaCl, 100 mM imidazole, 0.5 mM PMSF, 1 mM DTT, and 10 % glycerol. The enzyme is stable in this buffer at -20~70oC. In general, the enzyme is less stable when diluted. We doníŽt recommend any dilution of the enzyme.

Concentration:
LAE-E II-SUMO is supplied at the concentration of 0.5ug/ul (Cat # P002:100ug/tube,200ul/tube; Cat # P011: 50ug/tube,100ul/tube).

Shipping/Storage:
The enzyme is shipped on ice and must be stored at -20oC or lower.

Legal consideration: FOR RESEARCH USE ONLY.

 

 

Left: Human topo I (S35Met-labeled)control.
Right: Sumoylated human topo I (S35Met-labeled)
(7.5 ug/ml E1, 50 ug/ml E2, 50 ug/ml sumo1, 37oC 30min).

 


[1] Also called UBE2I or UBCE9.